CpG Methylation Analysis by Pyrosequencing Benchmarks and Application

Predicting CpG Islands and DNA Methlation in the Cow Genome Using DNA Microarray Meta-Analysis and Genome Wide Scanning

DNA methylation is a type of epigenetic changes that directly affects DNA. In mammals, DNA methylation is essential for fetal development and stem cell differentiation and this phenomenon essentially occurs within the CpG islands. In this study, two methods were used to study the DNA methylation profile of cow genome. In the first method, the DNA methylation profile of the differentially expres...

Analysis and quantification of multiple methylation variable positions in CpG islands by Pyrosequencing.

Serial pyrosequencing for quantitative DNA methylation analysis.

BioTechniques 721 In mammals, DNA methylation is a genetically programmed DNA modification and occurs almost exclusively at the 5-position of the pyrimidine ring of cytosines. Cytosine methylation is essential for mammalian embryogenesis and development, implicated in X inactivation, and influences gene expression of the corresponding genes, giving rise to distinct expressions patterns in diffe...

Systematic cross-validation of 454 sequencing and pyrosequencing for the exact quantification of DNA methylation patterns with single CpG resolution

BACKGROUND New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-validation of 454 sequencing and conventional pyrosequencing, both of which off...

Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

The existence of cytosine methylation in mammalian mitochondrial DNA (mtDNA) is a controversial subject. Because detection of DNA methylation depends on resistance of 5'-modified cytosines to bisulfite-catalyzed conversion to uracil, examined parameters that affect technical adequacy of mtDNA methylation analysis. Negative control amplicons (NCAs) devoid of cytosine methylation were amplified t...